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Case Report Pages 33-41
Abstract: It is feasible to beat extreme occurrences of male element fruitlessness, for example, azoospermia, with the utilization of intracytoplasmic sperm infusion. The chance for beset guys to become natural guardians is made accessible by the recovery of the male gamete from the epididymis or the testis. The reason for this study is to analyze the latest discoveries about the careful sperm recovery methods that might be utilized related to in vitro treatment (ICSI). We present a verifiable survey of the development of careful sperm recovery techniques, remembering its sign for both azoospermic and non-azoospermic men, as well as the specialized elements of each methodology.
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Case Report Pages 30-32
Abstract: Structural integrity and function of the plasma membrane of the cell defines the viability of spermatozoa. HOS (Hypo-Osmotic Swelling) test is considered as Benchmark test for checking the vitality of spermatozoa. The constituents of HOS test are 0.735g of sodium citrate dihydrate and 1.351g of D-fructose in 100 ml of purified water has an osmolarity of 150 mOsm. The basic principle of HOS test is spermatozoa when subjected to HOS solution, Sperm tails expand and coils based on plasma membrane integrity. Coiled tails are considered as vital spermatozoa. Spermatozoa which do not show any coiling due to loss of plasma membrane integrity are considered as dead spermatozoa.
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