Table 1: Overview of the experimental conditions for the Nitrate toxicity tests with surface water and groundwater crustaceans.
|
Acute tests (24h) |
|
|
|
|
Nitrate (mg/l) |
G. fossarum |
N. casparyi |
P. slavus |
|
0 |
R: 2; N: 4 |
R: 2; N: 4 |
R: 2; N: 4 |
|
50 |
R: 2; N: 4 |
R: 2; N: 4 |
R: 2; N: 4 |
|
100 |
R: 2; N: 4 |
R: 2; N: 4 |
R: 2; N: 4 |
|
Chronic tests (5 w) |
|
|
|
|
Concentration levels |
10 ℃: 0,5,25,50 |
10 ℃: 0,50,100 |
10 ℃: 0,50,100 |
|
|
18 ℃: 0,25,50,100 |
|
|
|
Replicates (R), Animals (N) |
R: 5; N: 5 |
R: 3; N: 3 |
R: 3; N: 3 |
|
Food |
Alder (3 cm2) |
Ash (0.25 cm2), FPOM |
Ash (0.25 cm2), FPOM |
|
Substrate |
Pebbles |
Pebbles |
Pebbles |
|
Aeration |
Yes |
No |
No |
|
Measured concentrations |
Mean (SD) |
|
|
|
Control |
1.7 (0.9) |
2.3 (0.9) |
2.3 (0.9) |
|
5 |
4.4 (0.4) |
|
|
|
25 |
26.9 (4.2) |
|
|
|
50 |
46.5 (3.2) |
44.3 (0.9) |
44.3 (0.9) |
|
100 |
119.5 (6.5) |
110 (5.3) |
110 (5.3) |