Table
1: Products
and supplies for culture of mouse pulmonary microvascular endothelial cells.
|
Product |
Supplier |
Supplier address |
Product number |
|
Dulbeccos’s modified Eagles’s
medium (DMEM), powder, high glucose |
Thermo Fisher Scientific |
West
Columbia, SC, USA |
12100-046 |
|
Fetal
bovine serum (FBS) |
Sigma-Aldrich |
St.
Louis, MO, USA |
SV30014.03 or
F0926 |
|
Endothelial
basal medium-2 (EBM-2) and BulletKit™ |
Lonza |
Allendale,
NJ, USA |
CC-3156 |
|
Penicillin
and streptomycin |
Thermo Fisher Scientific |
As
above |
15140122 |
|
Fibronectin |
Thermo Fisher Scientific |
As
above |
33016015 |
|
Gelatin |
Sigma-Aldrich |
As
above |
G1890 |
|
Collagen
type I (PureCol) |
Advanced
BioMatrix |
San
Diego, CA, USA |
5005-100
ML |
|
Hank’s
balanced salt solution (HBSS) |
Thermo Fisher Scientific |
As
above |
14185052 |
|
Phosphate-buffered
saline (PBS) without Ca2+ and Mg2+ |
Thermo Fisher Scientific |
As
above |
14190250 |
|
Bovine
serum albumin (BSA) |
Thermo Fisher Scientific |
As
above |
A9647 |
|
Collagenase
type I |
Worthington
Biochemical |
Lakewood,
NJ, USA |
LS004196 |
|
Dynabeads® sheep anti-rat IgG |
Thermo Fisher Scientific |
As
above |
11035 |
|
DynaMag® spin magnet (MPC) |
Thermo Fisher Scientific |
As
above |
12320D |
|
Platelet
endothelial cell adhesion molecule (PECAM-1; CD31) *discontinued
at TFS May
consider other company |
Santa
Cruz |
Santa
Cruz, CA, USA |
Sc-376764 |
|
Cell
strainer (70 µm) |
Thermo Fisher Scientific |
As
above |
22-363-548 |
|
Sterilization
filter (0.22 µm) |
Thomas
Scientific |
Swedesboro,
NJ, USA |
1226S77 |
|
0.05%
Trypsin-EDTA |
Thermo Fisher Scientific |
As
above |
25300-054 |
|
Accutase |
Innovative
Cell Technologies |
San
Diego, CA, USA |
#AT-104 |
|
ECIS
array |
Applied
BioPhysics |
Troy,
NY, USA |
8W1E
PC |