Table 1: Products and supplies for culture of mouse pulmonary microvascular endothelial cells.

Product

Supplier

Supplier address

Product number

Dulbeccos’s modified Eagles’s medium (DMEM), powder, high glucose

Thermo Fisher Scientific

West Columbia, SC, USA

12100-046

Fetal bovine serum (FBS)

Sigma-Aldrich

St. Louis, MO, USA

SV30014.03

or F0926

Endothelial basal medium-2 (EBM-2) and BulletKit

Lonza

Allendale, NJ, USA

CC-3156

Penicillin and streptomycin

Thermo Fisher Scientific

As above

15140122

Fibronectin

Thermo Fisher Scientific

As above

33016015

Gelatin

Sigma-Aldrich

As above

G1890

Collagen type I (PureCol)

Advanced BioMatrix

San Diego, CA, USA

5005-100 ML

Hank’s balanced salt solution (HBSS)

Thermo Fisher Scientific

As above

14185052

Phosphate-buffered saline (PBS) without Ca2+ and Mg2+

Thermo Fisher Scientific

As above

14190250

Bovine serum albumin (BSA)

Thermo Fisher Scientific

As above

A9647

Collagenase type I

Worthington Biochemical

Lakewood, NJ, USA

LS004196

Dynabeads® sheep anti-rat IgG

Thermo Fisher Scientific

As above

11035

DynaMag® spin magnet (MPC)

Thermo Fisher Scientific

As above

12320D

Platelet endothelial cell adhesion molecule (PECAM-1; CD31) *discontinued at TFS

May consider other company

Santa Cruz

Santa Cruz, CA, USA

Sc-376764

Cell strainer (70 µm)

Thermo Fisher Scientific

As above

22-363-548

Sterilization filter (0.22 µm)

Thomas Scientific

Swedesboro, NJ, USA

1226S77

0.05% Trypsin-EDTA

Thermo Fisher Scientific

As above

25300-054

Accutase

Innovative Cell Technologies

San Diego, CA, USA

#AT-104

ECIS array

Applied BioPhysics

Troy, NY, USA

8W1E PC