Table 2: Influence of storage medium on survival, virulence and aggressiveness of Phytophthora species stored for 5 years.
Medium1 |
Viability Test |
Pod necrosis (cm²) |
Aggressive ness |
Leaf symptom rating |
Zoospores (x 104)/ml suspension |
|
Tetrazolium |
Agar Plate |
|||||
SDW (Pm) |
+ |
+ |
4.1bc |
0.7a |
0.4b |
188 |
SDW (Pp) |
+ |
+ |
4.5b |
0.5b |
0.5b |
213 |
SSS (Pm) |
+ |
- |
5.4a |
0.7a |
0.4b |
113 |
SSS (Pp) |
+ |
+ |
5.5a |
0.4b |
3.1a |
513 |
Pre-storage (initial values) |
|
|
|
|
|
|
Pm |
+ |
+ |
8.1 |
NA |
3.2 |
151 |
Pp |
+ |
+ |
7.7 |
NA |
2.5 |
375 |
Fungi (P. megakarya, Pm and P. palmivora, Pp) stored from 15/3/2014 to 17/03/2019. SDW: Sterile Distilled Water and SSS: Sterilized Soil Suspension; +: Present; -: Absent. Aggressiveness values less than 1: loss of aggressiveness; value above 1: increase in aggressiveness. Leaf symptom rating (0: Absence of symptoms; 1: Initiation of small necrotic spots; 2: large number of small necrotic spots; 3: Coalescence of brown-necrotic spots, 4: Large, brown-lesions and 5: Large, uniform, brown-lesions; often expanding).