Table 2: Influence of storage medium on survival, virulence and aggressiveness of Phytophthora species stored for 5 years.

Medium1

Viability Test

Pod necrosis (cm²)

Aggressive ness

Leaf symptom rating

Zoospores (x 104)/ml suspension

Tetrazolium

Agar Plate

SDW (Pm)

+

+

4.1bc

0.7a

0.4b

188

SDW (Pp)

+

+

4.5b

0.5b

0.5b

213

SSS (Pm)

+

-

5.4a

0.7a

0.4b

113

SSS (Pp)

+

+

5.5a

0.4b

3.1a

513

Pre-storage (initial values)

 

 

 

 

 

 

Pm

+

+

8.1

NA

3.2

151

Pp

+

+

7.7

NA

2.5

375

Fungi (P. megakarya, Pm and P. palmivora, Pp) stored from 15/3/2014 to 17/03/2019. SDW: Sterile Distilled Water and SSS: Sterilized Soil Suspension; +: Present; -: Absent. Aggressiveness values less than 1: loss of aggressiveness; value above 1: increase in aggressiveness. Leaf symptom rating (0: Absence of symptoms; 1: Initiation of small necrotic spots; 2: large number of small necrotic spots; 3: Coalescence of brown-necrotic spots, 4: Large, brown-lesions and 5: Large, uniform, brown-lesions; often expanding).