Table 3: Piperidine alkaloids in in vitro grown plants of A. gariepensis, A. globuligemma and A. viguieri.
Species | Sample | Rt (min) | Match (%) | Name | Area (%) of extractf |
A. gariepensisd | Leaf | - | - | No piperidine alkaloids | - |
Root | 4.55 | a | (Coniine) | Trace | |
5.05 | a | (N-Methylconiine) | Trace | ||
A. globuligemma | Leaf | 5.85c | b | Coniine | 0.53 |
6.40c | b | N-Methylconiine | 10.93 | ||
6.50c | b | γ-Coniceine | 4.24 | ||
Root | - | - | No piperidine alkaloids | - | |
A. viguierie | Leaf | 4.55 | b | Coniine | 4.98 |
5.05 | b | N-Methylconiine | 1.48 | ||
5.25 | 90 | γ-Coniceine | 1.89 | ||
Root | 4.55 | 86 | Coniine | 0.26 | |
5.05 | a | (N-Methylconiine) | Trace | ||
5.25 | a | (γ-Coniceine) | Trace |
Traces in brackets. aIdentified on the basis of the base peak fragment present (coniine m/z 84, γ-coniceine m/z 97, N-methylconiine m/z 98); bIdentified by comparison to known spectra ([43] Palisade Complete 600K Mass Spectral Library); cDifferent GC-MS analysis conditions; dCell lines A-C of A. gariepensis did not contain piperidine alkaloids; eCell lines D-F of A. viguieri did not contain piperidine alkaloids; fThe repeatability for coniine standard is 2.7% RSD (n = 6).