Table 2: Effect of drugs on the total percent distribution of well-fed fish in the different colored arms of the T-maze tank during each 5 minute increment the 30 minutes recording.

Green arm Red arm Tail
Time/Mins C A N AN C A N AN C A N AN
5 4 ± 1 71 ± 8 22 ± 6 60 ± 8 0 ± 0 2 ± 2 0 6 ± 3 96 ± 1 27 ± 8 78 ± 6 34 ± 7
10 17 ± 5 73 ± 7 22 ± 4 61 ± 4 3 ± 2 5 ± 5 0 8 ± 3 80 ± 4 22 ± 9 78 ± 4 31 ± 6
15 21 ± 3 69 ± 9 21 ± 5 47 ± 8 4 ± 2 6 ± 5 0 6 ± 2 75 ± 4 25 ± 9 79 ± 5 46 ± 9
20 30 ± 4 56 ± 6 21 ± 3 39 ± 9 8 ± 4 8 ± 5 0 5 ± 2 62 ± 4 36 ± 5 79 ± 3 56 ± 10
25 39 ± 3 53 ± 4 20 ± 6 28 ± 2 8 ± 6 13 ± 6 0 6 ± 3 53 ± 4 34 ± 5 80 ± 3 66 ± 4
30 39 ± 6 52 ± 5 22 ± 9 17 ± 4 13 ± 7 16 ± 7 0 4 ± 2 48 ± 5 32 ± 7 80 ± 9 79 ± 4

(Values expressed as Mean ± SD of 3 replicates with 6 fish in each replicates, a total of 18 fish in each group).

C = Control; A = Alcohol; N = Nicotine; AN = Alcohol + Nicotine

One way ANOVA Zebrafish percentage distribution in the green arm of the T-maze tank
Within the group
Control (C) Alcohol (A) Nicotine (N) Alcohol + Nicotine (AN)
5 to 30 min [F = 2,15 = 0.02, p = 1]
With increasing time [F = 1,4 = ]
5 vs. 10 min [567, p < 0.01]
10 vs. 15 min [7, p = 0.06]
15 vs. 20 min [15, p = 0.02]
20 vs. 25 min [25, p = 0.01]
25 vs. 30 min [1, p = 0.4]
5 to 30 min [F = 2,15 = 0.17, p = 0.8]
With increasing time [F = 1,4 = ]
5 vs. 10 min [0.3, p = 0.6]
10 vs. 15 min [1, p = 0.4]
15 vs. 20 min [17, p = 0.02]
20 vs. 25 min [1, p = 0.4]
25 vs. 30 min [0.3, p = 0.6]
5 to 30 min [F = 2,15 = 4.1, p = 0.04]
With increasing time [F = 1,4 = ]
5 vs. 10 min [0, p = 1]
10 vs. 15 min [0.3, p = 0.6]
15 vs. 20 min [0.8, p = 0.8]
20 vs. 25 min [0.6, p = 0.5]
25 vs. 30 min [0, p = 1]
5 to 30 min [F = 2,15 = 0.02, p = 1]
With increasing time [F = 1,4 = ]
5 vs. 10 min [0.2, p = 0.7]
10 vs. 15 min [11, p = 0.03]
15 vs. 20 min [5, p = 0.09]
20 vs. 25 min [250, p < 0.01]
25 vs. 30 min [36, p < 0.01]

Between the groups at increasing time points of 5 minutes
C vs. A
[F = 1,4 = ]
C vs. N
[F = 1,4 = ]
C vs. AN
[F = 1,4 = ]
A vs. N
[F = 1,4 = ]
A vs. AN
[F = 1,4 = ]
N vs. AN
[F = 1,4 = ]
5-[687.6, p < 0.001]
10-[457.5, p < 0.001]
15-[308.7, p < 0.001]
20-[88.2, p < 0.001]
25-[53.8, p = 0.002]
30-[100.4, p < 0.001]
5-[109.3, p < 0.001]
10-[10.2, p = 0.03]
15-[0.01, p = 0.9]
20-[13.1, p = 0.02]
25-[543.3, p < 0.001]
30-[75.1, p < 0.001]
5-[401.5, p < 0.001]
10-[3396.3, p < 0.001]
15-[44.9, p = 0.003]
20-[23.2, p = 0.01]
25-[381.1, p < 0.001]
30-[150.1, p < 0.001]
5-[258.1, p < 0.001]
10-[312.0, p < 0.001]
15-[290.0, p < 0.001]
20-[213.7, p < 0.001]
25-[218.9, p < 0.001]
30-[166.5, p < 0.001]
5-[9.13, p = 0.04]
10-[21.5, p = 0.01]
15-[22.2, p = 0.01]
20-[64.9, p = 0.001]
25-[162.9, p < 0.001]
30-[265.4, p < 0.001]
5-[135.4, p < 0.001]
10-[710.3, p < 0.001]
15-[43.3, p = 0.03]
20-[203.0, p < 0.001]
25-[37.1, p = 0.004]
30-[1.1, p = 03]