Table 2: Effect of drugs on the total percent distribution of well-fed fish in the different colored arms of the T-maze tank during each 5 minute increment the 30 minutes recording.
| Green arm | Red arm | Tail | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time/Mins | C | A | N | AN | C | A | N | AN | C | A | N | AN |
| 5 | 4 ± 1 | 71 ± 8 | 22 ± 6 | 60 ± 8 | 0 ± 0 | 2 ± 2 | 0 | 6 ± 3 | 96 ± 1 | 27 ± 8 | 78 ± 6 | 34 ± 7 |
| 10 | 17 ± 5 | 73 ± 7 | 22 ± 4 | 61 ± 4 | 3 ± 2 | 5 ± 5 | 0 | 8 ± 3 | 80 ± 4 | 22 ± 9 | 78 ± 4 | 31 ± 6 |
| 15 | 21 ± 3 | 69 ± 9 | 21 ± 5 | 47 ± 8 | 4 ± 2 | 6 ± 5 | 0 | 6 ± 2 | 75 ± 4 | 25 ± 9 | 79 ± 5 | 46 ± 9 |
| 20 | 30 ± 4 | 56 ± 6 | 21 ± 3 | 39 ± 9 | 8 ± 4 | 8 ± 5 | 0 | 5 ± 2 | 62 ± 4 | 36 ± 5 | 79 ± 3 | 56 ± 10 |
| 25 | 39 ± 3 | 53 ± 4 | 20 ± 6 | 28 ± 2 | 8 ± 6 | 13 ± 6 | 0 | 6 ± 3 | 53 ± 4 | 34 ± 5 | 80 ± 3 | 66 ± 4 |
| 30 | 39 ± 6 | 52 ± 5 | 22 ± 9 | 17 ± 4 | 13 ± 7 | 16 ± 7 | 0 | 4 ± 2 | 48 ± 5 | 32 ± 7 | 80 ± 9 | 79 ± 4 |
(Values expressed as Mean ± SD of 3 replicates with 6 fish in each replicates, a total of 18 fish in each group).
C = Control; A = Alcohol; N = Nicotine; AN = Alcohol + Nicotine
| One way ANOVA Zebrafish percentage distribution in the green arm of the T-maze tank | |||
|---|---|---|---|
| Within the group | |||
| Control (C) | Alcohol (A) | Nicotine (N) | Alcohol + Nicotine (AN) |
| 5 to 30 min [F = 2,15 = 0.02, p = 1] With increasing time [F = 1,4 = ] 5 vs. 10 min [567, p < 0.01] 10 vs. 15 min [7, p = 0.06] 15 vs. 20 min [15, p = 0.02] 20 vs. 25 min [25, p = 0.01] 25 vs. 30 min [1, p = 0.4] |
5 to 30 min [F = 2,15 = 0.17, p = 0.8] With increasing time [F = 1,4 = ] 5 vs. 10 min [0.3, p = 0.6] 10 vs. 15 min [1, p = 0.4] 15 vs. 20 min [17, p = 0.02] 20 vs. 25 min [1, p = 0.4] 25 vs. 30 min [0.3, p = 0.6] |
5 to 30 min [F = 2,15 = 4.1, p = 0.04] With increasing time [F = 1,4 = ] 5 vs. 10 min [0, p = 1] 10 vs. 15 min [0.3, p = 0.6] 15 vs. 20 min [0.8, p = 0.8] 20 vs. 25 min [0.6, p = 0.5] 25 vs. 30 min [0, p = 1] |
5 to 30 min [F = 2,15 = 0.02, p = 1] With increasing time [F = 1,4 = ] 5 vs. 10 min [0.2, p = 0.7] 10 vs. 15 min [11, p = 0.03] 15 vs. 20 min [5, p = 0.09] 20 vs. 25 min [250, p < 0.01] 25 vs. 30 min [36, p < 0.01] |
| Between the groups at increasing time points of 5 minutes | |||||
|---|---|---|---|---|---|
| C vs. A [F = 1,4 = ] |
C vs. N [F = 1,4 = ] |
C vs. AN [F = 1,4 = ] |
A vs. N [F = 1,4 = ] |
A vs. AN [F = 1,4 = ] |
N vs. AN [F = 1,4 = ] |
| 5-[687.6, p < 0.001] 10-[457.5, p < 0.001] 15-[308.7, p < 0.001] 20-[88.2, p < 0.001] 25-[53.8, p = 0.002] 30-[100.4, p < 0.001] |
5-[109.3, p < 0.001] 10-[10.2, p = 0.03] 15-[0.01, p = 0.9] 20-[13.1, p = 0.02] 25-[543.3, p < 0.001] 30-[75.1, p < 0.001] |
5-[401.5, p < 0.001] 10-[3396.3, p < 0.001] 15-[44.9, p = 0.003] 20-[23.2, p = 0.01] 25-[381.1, p < 0.001] 30-[150.1, p < 0.001] |
5-[258.1, p < 0.001] 10-[312.0, p < 0.001] 15-[290.0, p < 0.001] 20-[213.7, p < 0.001] 25-[218.9, p < 0.001] 30-[166.5, p < 0.001] |
5-[9.13, p = 0.04] 10-[21.5, p = 0.01] 15-[22.2, p = 0.01] 20-[64.9, p = 0.001] 25-[162.9, p < 0.001] 30-[265.4, p < 0.001] |
5-[135.4, p < 0.001] 10-[710.3, p < 0.001] 15-[43.3, p = 0.03] 20-[203.0, p < 0.001] 25-[37.1, p = 0.004] 30-[1.1, p = 03] |