Table 1: Primers specific to multiplex Carbapenemase (blagene) with sequence and amplicon size (bp):
|
PCR name |
Sequence (5´-3´) |
Amplicon size (bp) |
|
Multiplex-1 |
|
|
|
blaVIM |
F-GATGGTGTTTGGTCGCATA R-CGAATGCGCAGCCCAG |
390 |
|
blaIMP |
F-TTGACACTCCATTTACDG R-GATYGAGAATTAAGCCACYCT
|
139
|
|
blaKPC |
F-CATTCAAGGGCTTTCTTGCTGC R-ACGACGGCATAGTCATTTGC |
538 |
|
Multiplex-2 |
|
|
|
blaGES |
F-AGTCGGCTAGACCGGAAAG R-TTTGTCCGTGCTCAGGAT |
399 |
|
blaOXA |
F-GCTTGATCGCCCTCGATT R- GATTTGCTCCGTGGCCGAAA |
281 |
|
blaNDM-1 |
F-ATGGAATTGCCCAATATTATGCAC R- TCAGCGCAGCTTGTCGGC |
813 |
|
Multiplex-3 |
|
|
|
BlaOXA-51 |
F- TAA TGC TTT GATCGG CCT TG R- TGG ATT GCA CTT CAT CTT GG |
353 |
|
blaOXA-23 |
F-GAT CGG ATT GGA GAA CCA GA R-ATT TCT GAC CGC ATT TCC AT |
501 |