Table 2: Experimental results of Chlorella vulgaris cultivation as a function of two independent variables, glycerol (Cgly) and corn steep liquor (CCSL), and their responses variables (Xm, Px, PTact, SFact and Fact/Pact).
| Days | Test | x1a | x2b | Cglyc (%) | Ccsld (%) | Xme(mg L-1) | Pxf (mg L-1 dia-1) | PTactg (U mL-1) | SFacth (U mg-1) | Fact (U mg-1)/Pact (U mg-1) |
| 6 | 1 | -1 | -1 | 0.5 | 0.5 | 881.2 | 146.0 | 13.00 | 226.6 | 1.314 |
| 6 | 2 | -1 | 1 | 0.5 | 1.5 | 1,325 | 220.0 | 17.40 | 158.6 | 0.703 |
| 9 | 3 | 1 | -1 | 1.5 | 0.5 | 680.7 | 75.00 | 18.09 | 467.1 | 1.992 |
| 5 | 4 | 1 | 1 | 1.5 | 1.5 | 1,165 | 232.0 | 12.15 | 590.2 | 1.416 |
| 8 | 5 | 1.414 | 0 | 1.7 | 1.0 | 1,200 | 150.0 | 17.58 | 662.6 | 1.907 |
| 9 | 6 | -1.414 | 0 | 0.3 | 1.0 | 1,250 | 139.0 | 16.11 | 183.2 | 1.031 |
| 6 | 7 | 0 | 1.414 | 1.0 | 1.7 | 1,260 | 213.0 | 16.96 | 198.8 | 0.904 |
| 5 | 8 | 0 | -1.414 | 1.0 | 0.3 | 650.0 | 130.0 | 19.22 | 313.6 | 1.065 |
| 8 | 9 | 0 | 0 | 1.0 | 1.0 | 1,430 | 179.0 | 20.69 | 153.8 | 1.383 |
| 8 | 10 | 0 | 0 | 1.0 | 1.0 | 1,480 | 185.0 | 21.21 | 261.8 | 1.602 |
| 8 | 11 | 0 | 0 | 1.0 | 1.0 | 1,430 | 179.0 | 24.03 | 242.2 | 1.754 |
| 8 | 12 | 0 | 0 | 1.0 | 1.0 | 1,480 | 185.0 | 20.51 | 143.0 | 1.358 |
| 7 | 13i | - | - | - | - | 698.7 | 99.80 | 5.000 | UN | UN |
| - | 14j | 1.450 | 1.600 | 0.9 | 1.2 | 1,520 | - | 21.00 | - | 1.517 |
| - | 15l | 1.530 | 1.445 | 1.1 | 0.9 | 1,384 | - | 21.70 | - | 1.796 |
| - | 16m | 2.000 | 1.400 | 2.0 | 0.8 | 666.1 | - | 12.00 | - | 2.100 |
ax1: Variable coded for Cgly; bx2: Variable coded for Ccsl; cCgly: Glycerol concentration (%); dCcsl: Corn steep liquor concentration (%); eXm: Maximum biomass concentration; fPx: Cell Productivity; gPact: Protease activity; hFact: Fibrinolytic activity; iAutotrophic conditions; UN: Unrealized. Values calculated by regression equation of JXm, lPTact, mFact/Pact.