Table 2: Development of LAMP assay.

Components Stock Concentration Working Concentration Required volume/reaction

Double distilled water1.0 ml 8.8 l

Betaine 1.6 M

Tris-HCl(pH8.8) 40 mM

KCl 20 mM 2x Reaction Mix (RM) 12.5 l

MgSO4

RM 16 mM

(NH4)2SO4 20 mM

Tween 20 0.2%

DNTPs 2.8 mM each

F3 primer 100 mM 5.0 pmol 0.05 l

B3 primer 100 mM 5.0 pmol 0.05 l

FIP 100 mM 40 pmol 0.2 l

BIP 100 mM 40 pmol 0.2 l

LF 100 mM 20 pmol 0.1 l

LB 100 mM 20 pmol 0.1 l

Calcein 1 l

8 units of Bst DNA polymerase 1 l

Template DNA 1 l

Total reaction volume 25 l