Table 2: Development of LAMP assay.

Components Stock Concentration Working Concentration Required volume/reaction

Double distilled water1.0 ml 8.8 µl

Betaine 1.6 M

Tris-HCl(pH8.8) 40 mM

KCl 20 mM 2x Reaction Mix (RM) 12.5 µl

MgSO₄

RM 16 mM

(NH₄)₂SO₄ 20 mM

Tween 20 0.2%

DNTPs 2.8 mM each

F3 primer 100 mM 5.0 pmol 0.05 µl

B3 primer 100 mM 5.0 pmol 0.05 µl

FIP 100 mM 40 pmol 0.2 µl

BIP 100 mM 40 pmol 0.2 µl

LF 100 mM 20 pmol 0.1 µl

LB 100 mM 20 pmol 0.1 µl

Calcein 1 µl

8 units of Bst DNA polymerase 1 µl

Template DNA 1 µl

Total reaction volume 25 µl