Table 1:The table shows the number of vital cells after two hours of exposure to increasing concentrations of Vitamin C in the culture medium. The cell lines used in this experiment are variants of human myeloid leukemia cells, and include: HL60, NB4, K562, U937, NB4-R1, NB4/As). It is evident that the total number of cells in culture decreases by increasing the concentration of Vitamin C in the culture medium.
HL60 (2 h) | NB4 (2 h) | K562 (2 h) | U937 (2 h) | NB4-R1 (2 h) | NB4/As (2 h) | ||
---|---|---|---|---|---|---|---|
Exp. 1 | Contr. | 471 | 912 | 663 | 1189 | 337 | 819 |
VC 0.5 | 296 | 680 | 669 | 476 | 82.3 | 42.7 | |
VC 1 | 108 | 496 | 628 | 245 | 39.7 | 31.9 | |
VC 3 | 22.6 | 163 | 226 | 56.4 | 47 | 32.2 | |
VC 5 | 15.1 | 143 | 82 | 30.2 | 35 | 48.4 | |
VC 7 | 6.15 | 85.4 | 32.2 | 10.6 | 24.6 | 17.6 | |
Exp. 2 | Contr. | 869 | 1,020 | 694 | 829 | 936 | 958 |
VC 0.5 | 423 | 956 | 399 | 704 | 624 | 823 | |
VC 1 | 349 | 887 | 445 | 585 | 560 | 674 | |
VC 3 | 217 | 337 | 200 | 232 | 335 | 581 | |
VC 5 | 143 | 74.6 | 111 | 118 | 344 | 402 | |
VC 7 | 89.5 | 147 | 35.2 | 93.7 | 255 | 329 | |
Exp. 3 | Contr. | 843 | 1,130 | 1,020 | 969 | 967 | 859 |
VC 0.5 | 545 | 924 | 660 | 716 | 678 | 722 | |
VC 1 | 438 | 835 | 507 | 689 | 668 | 649 | |
VC 3 | 343 | 395 | 113 | 480 | 551 | 499 | |
VC 5 | 218 | 346 | 35.2 | 411 | 443 | 453 | |
VC 7 | 181 | 241 | 17.6 | 320 | 380 | 414 |
C = control (untreated) sample; VC = Vitamin C; VC 0.5 mM, VC 1 mM, VC 3 mM, VC 5 mM = Vitamin C at 0.5, 1, 3, and 5 millimoles in the culture medium.