Table 2:Analysis of variance for clotting activity along the experimental assay.

Source Sum of squares df Mean square F value P - value Prob > F
Model 32,438.645 3 10,812.88 22.75 0.0056
A-Enzyme 14,938.56 1 14,938.56 31.43 0.005
B-CaCl2 14,577.78 1 14,577.78 30.67 0.0052
BC-CaCl2-Temperature 2922.3 1 2922.3 6.15 0.0682
Residual 1901.3 4 3475.33
Total 34,339.95 7

p-value are statistically significant at 95% of confidence level.