Table 1:Code and parameters used in experimental design. The results of clotting activity using donkey milk by cyprosin were obtained in duplicated.
| Run number | Enzyme | CaCl2 (%) w/v | Temperature (℃) | Clotting strength (U) | Average clotting strength (U) | Standard deviation | |
|---|---|---|---|---|---|---|---|
| 1 | Cyprosin (-1) | 0.01 (-1) | 32 (-1) | 235.3 | 238.1 | 236.7 | ± 1.4 |
| 2 | Microbial rennet (+1) | 0.01 (-1) | 32 (-1) | 166.7 | 169.5 | 168.1 | ± 1.4 |
| 3 | Cyprosin (-1) | 0.1 (+1) | 32 (-1) | 333.3 | 444.4 | 388.9 | ± 55.6 |
| 4 | Microbial rennet (+1) | 0.1 (+1) | 32 (-1) | 266.7 | 259.7 | 263.2 | ± 3.5 |
| 5 | Cyprosin (-1) | 0.01 (-1) | 35 (+1) | 277.8 | 285.7 | 281.7 | ± 4.0 |
| 6 | Microbial rennet (+1) | 0.01 (-1) | 35 (+1) | 181.8 | 178.6 | 180.2 | ± 1.6 |
| 7 | Cyprosin (-1) | 0.1 (+1) | 35 (+1) | 298.5 | 307.7 | 303.1 | ± 4.6 |
| 8 | Microbial rennet (+1) | 0.1 (+1) | 35 (+1) | 250 | 256.4 | 253.2 | ± 3.2 |