Table 2: Primers used to evaluate the expression levels of 12 related genes in transgenic cucumber seedlings expressing the CGMMV-MP.

Primer name

Sequence (5' to 3')

Product length

Tubulin (reference gene)

Forward,GCGTTTGTCTGTTGACTATG

232 bp

Reverse, GGATACAAGACGGTTGAGG

 

Cytochrome b6-f complex

Foward, GCCACCACCTTCATCATCG

238 bp

Reverse, GGAAGAGAAGACACAAATGA

 

Cysteine synthase

Foward, GCCATCTTTGATCGAAAGACTAG

222 bp

Reverse, GAAACATGAAGGTTTTGAGCCG

 

Disulfide isomerase

Foward, GAGCAAGCCCTTTTGTGAAG

213 bp

Reverse, GATTCCCTGGATTTCGTGTCG

 

Catalase (CAT)

Foward, GATAGAATGGCCGGAGGATG

231 bp

Reverse, GGAGCATTAACAGGCAACTG

 

Cucumber peeling cupredoxin

Foward, GACTTGGGATTCCTGACAAAG

215 bp

Reverse, GCAGAGAAGAAATCACCGTG

 

NADH-quinone oxidoreductase subunit K

Foward, GTCTCGACTTCTTCGCTATG

226 bp

Reverse, GTCGTATTTCTTTGGGTTGG

 

NADH-quinone oxidoreductase subunit J

Foward, GAATGTGTGCTTTGAAGGG

229 bp

Reverse, GGTTGTAACGATGATGCG

 

Histone H4

Foward, GAAGGGAACCATGACAACCA

220 bp

Reverse, GAGGTCAACAACTGAAACG

 

Pathogen regulatory proteins CuPi1

Foward, GCTCAAGACTCACCTCAAG

201 bp

Reverse, GGCTGATAAGTCTGGGCTG

 

Phloem protein (PP2)

Foward, GAAAATTGACGTGGCACAG

235 bp

Reverse, GATCGTAAACCAAACATCTCG

 

Cytochrome oxidase

Foward, GTCATTCCCATTTAGGTCCG

222 bp

Reverse, GACCGAATTGGTACGGAAG

 

Thaumatin-like protein

Foward, GCCTTATGTGGTAGTGTTCG

172 bp

Reverse, GATCTGAAGTCTACGAGCATG