Table
2: Primers
used to evaluate the expression levels of 12 related genes in transgenic
cucumber seedlings expressing the CGMMV-MP.
|
Primer name |
Sequence (5' to
3') |
Product length |
|
Tubulin (reference gene) |
Forward,GCGTTTGTCTGTTGACTATG |
232 bp |
|
Reverse, GGATACAAGACGGTTGAGG |
|
|
|
Cytochrome b6-f complex |
Foward, GCCACCACCTTCATCATCG |
238 bp |
|
Reverse, GGAAGAGAAGACACAAATGA |
|
|
|
Cysteine synthase |
Foward, GCCATCTTTGATCGAAAGACTAG |
222 bp |
|
Reverse, GAAACATGAAGGTTTTGAGCCG |
|
|
|
Disulfide isomerase |
Foward, GAGCAAGCCCTTTTGTGAAG |
213 bp |
|
Reverse, GATTCCCTGGATTTCGTGTCG |
|
|
|
Catalase (CAT) |
Foward, GATAGAATGGCCGGAGGATG |
231 bp |
|
Reverse, GGAGCATTAACAGGCAACTG |
|
|
|
Cucumber peeling cupredoxin |
Foward, GACTTGGGATTCCTGACAAAG |
215 bp |
|
Reverse, GCAGAGAAGAAATCACCGTG |
|
|
|
NADH-quinone oxidoreductase subunit K |
Foward, GTCTCGACTTCTTCGCTATG |
226 bp |
|
Reverse, GTCGTATTTCTTTGGGTTGG |
|
|
|
NADH-quinone oxidoreductase subunit J |
Foward, GAATGTGTGCTTTGAAGGG |
229 bp |
|
Reverse, GGTTGTAACGATGATGCG |
|
|
|
Histone H4 |
Foward, GAAGGGAACCATGACAACCA |
220 bp |
|
Reverse, GAGGTCAACAACTGAAACG |
|
|
|
Pathogen regulatory proteins CuPi1 |
Foward, GCTCAAGACTCACCTCAAG |
201 bp |
|
Reverse, GGCTGATAAGTCTGGGCTG |
|
|
|
Phloem protein (PP2) |
Foward, GAAAATTGACGTGGCACAG |
235 bp |
|
Reverse, GATCGTAAACCAAACATCTCG |
|
|
|
Cytochrome oxidase |
Foward, GTCATTCCCATTTAGGTCCG |
222 bp |
|
Reverse, GACCGAATTGGTACGGAAG |
|
|
|
Thaumatin-like protein |
Foward, GCCTTATGTGGTAGTGTTCG |
172 bp |
|
Reverse, GATCTGAAGTCTACGAGCATG |
|