The reagents and conditions of PCR.

Table 2: The reagents and conditions of PCR.

Reagents	Single dose(μl)
Molecular Biology Grade Water	35.2
10 × PFX buffer	5
MgSO₄	2
dNTPs (10 mM each)	2
Fusion Primer A (10 μM)	2
Fusion Primer B (10 μM)	2
PFX polymerase	0.8

PCR conditions: 94 ℃, 3 min; (94 ℃, 30 s; 50 ℃, 30 s; 70 ℃, 45 s) ^*30cycle; 70 ℃, 7 min; 10 ℃.