Table 2: Chemical extractions and various reactions parameters with % oil yields [11].

Method of Extraction and reference

Temp. of reaction

pH of reaction

Reaction time (hours)

Oil yield (%)

n - hexane oil extraction (Soxhelt apparatus) by Gubitz, et al., Forson, et al. [4]

-

-

24

95-99

1st Acetone, 2nd n-hexane by Augustus, et al.

-

-

48

Aqueous Oil Extraction (AOE) by Winkler, et al.

-

-

2

38

Shah, et al.

50

9

6

38

AOE with 10 mins ultrasonication as pretreatment by Shah, et al.

50

9

6

67

Aqueous Enzymatic Oil Extraction (AEOE) by Winkler, et al. (hemicellulase or cellulose)

60

4.5

2

73

AEOE (alkaline protease) by Winkler, et al.

60

7

2

86

Shah, et al.

50

9

6

64

AEOE (alkaline protease) with 5 mins ultrasonic as pretreatment by Shah, et al.

50

9

6

74

Three phase partitioning [19]

25

9

2

97