Table 1: List of gene specific primers for real-time PCR.
| Genes | Accession number | Sequences | Size (bp) | Tm (℃) | Ct |
|---|---|---|---|---|---|
| miR-199a | - | F: GCC ATC CCA GTG TTC AGA CT | 118 | 60 | 40 |
| R: ACT GTA CAT GTC CCA GCC TC | |||||
| PPARγ2 | - | F: TCG CTG ATG CAC TGC CTA TG | 103 | 60 | 40 |
| R: GAG AGG TCC ACA GAG CTG ATT | |||||
| C/EBPα | NM-007678 | F: CAA GAA CAG CAA CGA GTA CCG | 124 | 60 | 40 |
| R: GTC ACT GGT CAA CTC CAG CAC | |||||
| aP2 | NM-024406 | F: ATC AGC GTA AAT GGG GAT TTG G | 105 | 60 | 40 |
| R: GTC TGC GGT GAT TTC ATC GAA | |||||
| SREBP-1c | NM-011480.3 | F: GCG CTA CCG GTC TTC TAT CA | 113 | 60 | 40 |
| R: GGA TGT AGT CGA TGG CCT TG | |||||
| β-actin (house-keeping genes) | NM-007393.3 | F: ACC CCA GCC ATG TAC GTA GC | 101 | 60 | 40 |
| R: GTG TGG GTG ACC CCG TCT C |