Table 2: Summary of validation parameter.
| Sr. no. | Parameters | Results |
| 1 | Linearity and Range | |
| Range (μg/ml) | 0.5-30 | |
| Correlation coefficient (R2) | 0.999 | |
| 2 | Precision (% RSD) | |
| Intra-day (n = 3) | 0.18-1.31 | |
| Inter-day (n = 3) | 0.31-1.14 | |
| Repeatability (n = 6) | 0.853 | |
| 3 | LOD (μg/ml) | 0.1 |
| 4 | LOQ (μg/ml) | 0.5 |
| 5 | Accuracy (Mean ± SD) (n = 3) | |
| 50% target concentration | 98.93 ± 0.43 | |
| 100% target concentration | 99.06 ± 0.15 | |
| 150% target concentration | 99.44 ± 0.54 | |
| 6 | Specificity | Specific |