Table 2: Method validation parameters for HPLC-UV method optimized for the determination of CIP in water sample.
|
Method Validation Parameters |
CIP |
|
Linear range (µg/ml) |
50-500 |
|
Linearity (R2) |
0.9989 |
|
LOD (µg/ml) |
61.2 |
|
LOQ (µg/ml) |
185.5 |
|
Accuracy (Recovery %) 50 µg 200 µg 300 µg |
22.99% 102.6% 151.62% |
|
Intraday assay precision, n = 3 (RSD %) 50 µg 200 µg 300 µg |
0.836 0.998 0.244 |
|
Inter day assay precision, n = 3 (RSD %) 50 µg 200 µg 300 µg |
0.832 1.11 0.755 |