Table 2: Validation data for determination of lamivudine by the proposed HPLC procedure.
|
Item |
LVD |
|
λ max (nm) |
280 |
|
Concentration range (µg/ml) |
5-150 |
|
Regression equation: |
|
|
Intercept(a) |
1752.70 |
|
Slope (b) |
19189.49 |
|
Variance of intercept (Sa2) |
3853.46 |
|
Variance around slope (Sb2) |
50.08 |
|
Correlation coefficient(r) |
0.99999 |
|
Variance (Sy/x) |
6495.06 |
|
Accuracy (mean ± SD %) |
98.99 ± 0.74% |
|
Precision (RSD %) |
0.26% |
|
Limit of detection (µg/ml) |
1.09 |
|
Limit of quantitation (µg/ml) |
3.6 |